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FIGURE 1 | Ultrastructural analysis of <t>Sporothrix</t> brasiliensis treated with silver nanocomposites. S. brasiliensis ATCC 5110 treated for 48 h with MIC50 of nanocomposites were analyzed by TEM. Untreated yeasts exhibited an intact structure: compact cell wall, continuous cytoplasmic membrane and electrodense (A,B). While yeasts exposed to AgNPs showed damage to the integrity of the membrane of cytoplasmic organelles and the cell membrane (white arrows) (C,D). Cells treated with AgNPs@Chi exhibited membrane detachment and cell surface disaggregation (E,F). Bars scale = 500 nm (A,C,E) and 200 nm (B,D,F).
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FIGURE 1 | Ultrastructural analysis of <t>Sporothrix</t> brasiliensis treated with silver nanocomposites. S. brasiliensis ATCC 5110 treated for 48 h with MIC50 of nanocomposites were analyzed by TEM. Untreated yeasts exhibited an intact structure: compact cell wall, continuous cytoplasmic membrane and electrodense (A,B). While yeasts exposed to AgNPs showed damage to the integrity of the membrane of cytoplasmic organelles and the cell membrane (white arrows) (C,D). Cells treated with AgNPs@Chi exhibited membrane detachment and cell surface disaggregation (E,F). Bars scale = 500 nm (A,C,E) and 200 nm (B,D,F).
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FIGURE 1 | Ultrastructural analysis of <t>Sporothrix</t> brasiliensis treated with silver nanocomposites. S. brasiliensis ATCC 5110 treated for 48 h with MIC50 of nanocomposites were analyzed by TEM. Untreated yeasts exhibited an intact structure: compact cell wall, continuous cytoplasmic membrane and electrodense (A,B). While yeasts exposed to AgNPs showed damage to the integrity of the membrane of cytoplasmic organelles and the cell membrane (white arrows) (C,D). Cells treated with AgNPs@Chi exhibited membrane detachment and cell surface disaggregation (E,F). Bars scale = 500 nm (A,C,E) and 200 nm (B,D,F).
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FIGURE 1 | Ultrastructural analysis of <t>Sporothrix</t> brasiliensis treated with silver nanocomposites. S. brasiliensis ATCC 5110 treated for 48 h with MIC50 of nanocomposites were analyzed by TEM. Untreated yeasts exhibited an intact structure: compact cell wall, continuous cytoplasmic membrane and electrodense (A,B). While yeasts exposed to AgNPs showed damage to the integrity of the membrane of cytoplasmic organelles and the cell membrane (white arrows) (C,D). Cells treated with AgNPs@Chi exhibited membrane detachment and cell surface disaggregation (E,F). Bars scale = 500 nm (A,C,E) and 200 nm (B,D,F).
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FIGURE 1 | Ultrastructural analysis of <t>Sporothrix</t> brasiliensis treated with silver nanocomposites. S. brasiliensis ATCC 5110 treated for 48 h with MIC50 of nanocomposites were analyzed by TEM. Untreated yeasts exhibited an intact structure: compact cell wall, continuous cytoplasmic membrane and electrodense (A,B). While yeasts exposed to AgNPs showed damage to the integrity of the membrane of cytoplasmic organelles and the cell membrane (white arrows) (C,D). Cells treated with AgNPs@Chi exhibited membrane detachment and cell surface disaggregation (E,F). Bars scale = 500 nm (A,C,E) and 200 nm (B,D,F).
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FIGURE 1 | Ultrastructural analysis of <t>Sporothrix</t> brasiliensis treated with silver nanocomposites. S. brasiliensis ATCC 5110 treated for 48 h with MIC50 of nanocomposites were analyzed by TEM. Untreated yeasts exhibited an intact structure: compact cell wall, continuous cytoplasmic membrane and electrodense (A,B). While yeasts exposed to AgNPs showed damage to the integrity of the membrane of cytoplasmic organelles and the cell membrane (white arrows) (C,D). Cells treated with AgNPs@Chi exhibited membrane detachment and cell surface disaggregation (E,F). Bars scale = 500 nm (A,C,E) and 200 nm (B,D,F).
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FIGURE 1 | Ultrastructural analysis of Sporothrix brasiliensis treated with silver nanocomposites. S. brasiliensis ATCC 5110 treated for 48 h with MIC50 of nanocomposites were analyzed by TEM. Untreated yeasts exhibited an intact structure: compact cell wall, continuous cytoplasmic membrane and electrodense (A,B). While yeasts exposed to AgNPs showed damage to the integrity of the membrane of cytoplasmic organelles and the cell membrane (white arrows) (C,D). Cells treated with AgNPs@Chi exhibited membrane detachment and cell surface disaggregation (E,F). Bars scale = 500 nm (A,C,E) and 200 nm (B,D,F).

Journal: Frontiers in Nanotechnology

Article Title: Silver Chitosan Nanocomposites are Effective to Combat Sporotrichosis

doi: 10.3389/fnano.2022.857681

Figure Lengend Snippet: FIGURE 1 | Ultrastructural analysis of Sporothrix brasiliensis treated with silver nanocomposites. S. brasiliensis ATCC 5110 treated for 48 h with MIC50 of nanocomposites were analyzed by TEM. Untreated yeasts exhibited an intact structure: compact cell wall, continuous cytoplasmic membrane and electrodense (A,B). While yeasts exposed to AgNPs showed damage to the integrity of the membrane of cytoplasmic organelles and the cell membrane (white arrows) (C,D). Cells treated with AgNPs@Chi exhibited membrane detachment and cell surface disaggregation (E,F). Bars scale = 500 nm (A,C,E) and 200 nm (B,D,F).

Article Snippet: Strain MIC50 (μg/ml) MIC90 (μg/ml) AgNPs AgNPs@Chi ITC AgNPs AgNPs@Chi ITC Sporothrix brasiliensis ATCC 5110 0.06 0.25 0.5 0.12 0.5 1 Sporothrix schenckii ATCC 1099-18 0.12 0.25 0.5 0.25 1 1 MIC, minimum inhibitory concentration; ITC, itraconazole.

Techniques: Membrane